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reporter plasmids for supertopflash  (Addgene inc)


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    Structured Review

    Addgene inc reporter plasmids for supertopflash
    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
    Reporter Plasmids For Supertopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/8x+supertopflash+reporter+plasmid/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/bio_rxiv__2025__08__02__668297-251-22-30
    Average 96 stars, based on 576 article reviews
    reporter plasmids for supertopflash - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics"

    Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics

    Journal: bioRxiv

    doi: 10.1101/2025.08.02.668297

    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
    Figure Legend Snippet: (A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.

    Techniques Used: Transfection, Construct

    Related Articles

    Transfection:

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling
    Article Snippet: After 1 h, membranes were washed again for 5x5 min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. TOPFlash reporter gene assays HEK293 cells or HEK293 ΔFZD1 − 10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20% of HiBiT-FZD constructs (if indicated), and LRP6 constructs as annotated, and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well at bottom plate. ..

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling.
    Article Snippet: After 1 h,membranes werewashed again for 5 × 5min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. TOPFlash reporter gene assays HEK293 cells or HEK293 ΔFZD1-10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20%of HiBiT-FZD constructs (if indicated), andLRP6 constructs as annotated (1% if notmentionedotherwise), and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well flat bottom plate. ..

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling
    Article Snippet: After 1 h, membranes were washed again for 5 × 5 min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. HEK293 cells or HEK293 ΔFZD 1-10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20% of HiBiT-FZD constructs (if indicated), and LRP6 constructs as annotated (1% if not mentioned otherwise), and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well flat bottom plate. ..

    Plasmid Preparation:

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling
    Article Snippet: After 1 h, membranes were washed again for 5x5 min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. TOPFlash reporter gene assays HEK293 cells or HEK293 ΔFZD1 − 10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20% of HiBiT-FZD constructs (if indicated), and LRP6 constructs as annotated, and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well at bottom plate. ..

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling.
    Article Snippet: After 1 h,membranes werewashed again for 5 × 5min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. TOPFlash reporter gene assays HEK293 cells or HEK293 ΔFZD1-10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20%of HiBiT-FZD constructs (if indicated), andLRP6 constructs as annotated (1% if notmentionedotherwise), and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well flat bottom plate. ..

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling
    Article Snippet: After 1 h, membranes were washed again for 5 × 5 min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. HEK293 cells or HEK293 ΔFZD 1-10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20% of HiBiT-FZD constructs (if indicated), and LRP6 constructs as annotated (1% if not mentioned otherwise), and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well flat bottom plate. ..

    Control:

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling
    Article Snippet: After 1 h, membranes were washed again for 5x5 min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. TOPFlash reporter gene assays HEK293 cells or HEK293 ΔFZD1 − 10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20% of HiBiT-FZD constructs (if indicated), and LRP6 constructs as annotated, and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well at bottom plate. ..

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling.
    Article Snippet: After 1 h,membranes werewashed again for 5 × 5min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. TOPFlash reporter gene assays HEK293 cells or HEK293 ΔFZD1-10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20%of HiBiT-FZD constructs (if indicated), andLRP6 constructs as annotated (1% if notmentionedotherwise), and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well flat bottom plate. ..

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling
    Article Snippet: After 1 h, membranes were washed again for 5 × 5 min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. HEK293 cells or HEK293 ΔFZD 1-10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20% of HiBiT-FZD constructs (if indicated), and LRP6 constructs as annotated (1% if not mentioned otherwise), and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well flat bottom plate. ..

    Construct:

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling
    Article Snippet: After 1 h, membranes were washed again for 5x5 min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. TOPFlash reporter gene assays HEK293 cells or HEK293 ΔFZD1 − 10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20% of HiBiT-FZD constructs (if indicated), and LRP6 constructs as annotated, and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well at bottom plate. ..

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling.
    Article Snippet: After 1 h,membranes werewashed again for 5 × 5min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. TOPFlash reporter gene assays HEK293 cells or HEK293 ΔFZD1-10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20%of HiBiT-FZD constructs (if indicated), andLRP6 constructs as annotated (1% if notmentionedotherwise), and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well flat bottom plate. ..

    Article Title: WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling
    Article Snippet: After 1 h, membranes were washed again for 5 × 5 min with TBS-T. Blots were developed using Clarity Western ECL substrate (Bio-Rad) following the instructions of the manufacturer in a ChemiDoc chemiluminescence reader (Bio-Rad). .. HEK293 cells or HEK293 ΔFZD 1-10 cells were transfected with 25% of an 8x SuperTOPFlash reporter plasmid (Addgene #12456), 5% of a pRL-TK control plasmid (Promega), 20% of HiBiT-FZD constructs (if indicated), and LRP6 constructs as annotated (1% if not mentioned otherwise), and were seeded at a density of 35,000 and 45,000 cells per well, respectively, into a PDL-coated white 96-well flat bottom plate. ..



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    Addgene inc reporter plasmids for supertopflash
    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
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    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for <t>SuperTopFlash</t> together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.
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    Image Search Results


    (A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.

    Journal: bioRxiv

    Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics

    doi: 10.1101/2025.08.02.668297

    Figure Lengend Snippet: (A): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each GFP tagged DVL1 construct or with a 1:1 stoichiometric ratio of two DVL1 constructs with the same total amount of DVL1 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (B): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each FLAG tagged DVL2 construct or with a 1:1 stoichiometric ratio of two DVL2 constructs with the same total amount of DVL2 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. (C): HEK293T cells were transiently transfected with firefly reporter plasmids for SuperTopFlash together with a fixed amount of each HA tagged DVL3 construct or with a 1:1 stoichiometric ratio of two DVL3 constructs with the same total amount of DVL3 constructs. Data represents average values of relative TOPFlash over FOPFlash ratios from one representative experiment performed in triplicate. Individual p values were calculated with student t-tests and denote the difference between DVL-WT and the other conditions without WNT stimulation, difference between each condition with or without stimulation was also calculated. An asterisk indicates a significant difference: *: p < 0.05; **: p < 0.005; ***: p < 0.0005; ****: p < 0.0001.

    Article Snippet: Cells were transfected using Lipofectamine 3000 (Invitrogen, Cat. No. L3000-008) with plasmids containing the wild type or variant DVL genes plus firefly reporter plasmids for SuperTopFlash (M50 Super 8x TOPFlash, Addgene plasmid #12456) and FOPFlash (M51 Super 8x FOPFlash, Addgene plasmid #12457) plasmid used as a negative control for “leaky” firefly luciferase transcription.

    Techniques: Transfection, Construct